Purification and Characterization of a Nylon-Degrading Enzyme

Purification and Characterization of Cellulose Degrading Enzyme from Newly Isolated Cellulomonas Sp

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning 110866, China * Department of Biotechnology, University of Sargodha, Sargodha 40100, Pakistan ** English Teaching Department, Shenyang Agricultural University, Shenyang 110866 Liaoning, China *** Bioscience and Biotechn...

Partial Purification and Characterization of 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria Harboring Alpha Ketoglutarate Dioxgenase Enzyme

The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...

Purification and characterization of an intracellular cycloalternan-degrading enzyme from Bacillus sp. NRRL B-21195.

A novel intracellular cycloalternan-degrading enzyme (CADE) was purified to homogeneity from the cell pellet of Bacillus sp. NRRL B-21195. The enzyme has a molecular mass of 125 kDa on SDS-PAGE. The pH optimum was 7.0, and the enzyme was stable from pH 6.0 to 9.2. The temperature optimum was 35 degrees C and the enzyme exhibited stability up to 50 degrees C. The enzyme hydrolyzed cycloalternan ...

Construction and Characterization of a Lux-Marked Phenanthrene Degrading Bacterium

Purification and Partial Characterization of a Thrombin-Like Enzyme (AH144) from Venom of Iranian Snake Agkistrodon Halys

The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...